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Molecular Biology and Biochemistry Protocols Package v2.0
************* THE APP HAS BEEN FIXED AND NOW WORKS! (17-FEB-2020)************
* Over 70 most common protocols and buffer recipes used in the Molecular Biology, Biochemistry and Cell Biology labs.
* NEW: The protocols can be edited in Word and saved.
* The program can be copied to SD card.
* No internet connection is required to use the program (100% offline).
* The package will continue to grow and regular updates will be provided for free.
* Note: The package can be uninstalled and refunded within the first 15 minutes of purchase (Android Market rules).
Table of Contents for v1.0 (Jan 2012 release):
DNA World:
Agarose gel electrophoresis and PCR Check
Colony PCR from yeast colonies (application: Y2H screens)
Touch-down PCR protocol
10 Rules of Primer Design
Restriction Digestion Setup
Qiagens PCR purification column
Ligation Reaction Setup
T-vector for cloning PCR products
Phenol-Chloroform DNA isolation
Yeast Transformation
CIP-treatment
Plasmid Prep (classical protocol)
Miniprep (Qiagen kit)
Silver Staining of DNA Gels
LB-Ampicillin Plates
Chemically Competent E.
coli Preparation
Transformation of the Chemically Competent E.
coli Cells
Transformation - 5min Shortcut for Ampicillin Resistant Plasmids
Chromatin Immunoprecipitation (ChIP) Protocol
RNA World:
siRNA resuspension
RNA isolation from cultured cells by using RNeasy kit (Qiagen)
RT-PCR with oligo(dT) primers
Protein World:
General IP protocol
Protein expression in E.
coli.
Purification of His-tagged proteins from E.
coli
SDS-PAGE
Coomassie Brilliant Blue Staining of Protein Gels
Silver Staining of Protein Gels
Western Blotting
Western Blot Stripping
Preparation of Enhanced ChemiLuminescence (ECL) Substrate Buffers
IP-kinase protocol for P81 paper assay
IP-kinase protocol for autoradiography
Preparation of SILAC media
Sample Preparation for phospho-SILAC (global phosphoproteomic analysis)
Phospho-SILAC Analysis using MAXQUANT
Cell World:
Freezing cells
Hoechst staining
Immunofluorescence
Propidium Iodide Staining of Cells for Cell Cycle Analysis by FACS
Transfection of cells using PEI
C.
elegans World:
Egg prep protocol
Myosin purification from C.
Elegans
Buffer and Solution Recipes:
PBS
TBS
TBST
5X SDS-PAGE Sample Buffer
6X Agarose Loading Buffer
40% acryl stock (29: 1) (for DNA gels)
20% paraformaldehyde stock
0.5M ATP stock
1M DTT
0.5M EDTA
50X TAE
10X TBE
TE
20X SSC
LB medium
SOC medium
M9 Buffer
Lysis / IP Buffer
************* THE APP HAS BEEN FIXED AND NOW WORKS! (17-FEB-2020)************
* Over 70 most common protocols and buffer recipes used in the Molecular Biology, Biochemistry and Cell Biology labs.
* NEW: The protocols can be edited in Word and saved.
* The program can be copied to SD card.
* No internet connection is required to use the program (100% offline).
* The package will continue to grow and regular updates will be provided for free.
* Note: The package can be uninstalled and refunded within the first 15 minutes of purchase (Android Market rules).
Table of Contents for v1.0 (Jan 2012 release):
DNA World:
Agarose gel electrophoresis and PCR Check
Colony PCR from yeast colonies (application: Y2H screens)
Touch-down PCR protocol
10 Rules of Primer Design
Restriction Digestion Setup
Qiagens PCR purification column
Ligation Reaction Setup
T-vector for cloning PCR products
Phenol-Chloroform DNA isolation
Yeast Transformation
CIP-treatment
Plasmid Prep (classical protocol)
Miniprep (Qiagen kit)
Silver Staining of DNA Gels
LB-Ampicillin Plates
Chemically Competent E.
coli Preparation
Transformation of the Chemically Competent E.
coli Cells
Transformation - 5min Shortcut for Ampicillin Resistant Plasmids
Chromatin Immunoprecipitation (ChIP) Protocol
RNA World:
siRNA resuspension
RNA isolation from cultured cells by using RNeasy kit (Qiagen)
RT-PCR with oligo(dT) primers
Protein World:
General IP protocol
Protein expression in E.
coli.
Purification of His-tagged proteins from E.
coli
SDS-PAGE
Coomassie Brilliant Blue Staining of Protein Gels
Silver Staining of Protein Gels
Western Blotting
Western Blot Stripping
Preparation of Enhanced ChemiLuminescence (ECL) Substrate Buffers
IP-kinase protocol for P81 paper assay
IP-kinase protocol for autoradiography
Preparation of SILAC media
Sample Preparation for phospho-SILAC (global phosphoproteomic analysis)
Phospho-SILAC Analysis using MAXQUANT
Cell World:
Freezing cells
Hoechst staining
Immunofluorescence
Propidium Iodide Staining of Cells for Cell Cycle Analysis by FACS
Transfection of cells using PEI
C.
elegans World:
Egg prep protocol
Myosin purification from C.
Elegans
Buffer and Solution Recipes:
PBS
TBS
TBST
5X SDS-PAGE Sample Buffer
6X Agarose Loading Buffer
40% acryl stock (29: 1) (for DNA gels)
20% paraformaldehyde stock
0.5M ATP stock
1M DTT
0.5M EDTA
50X TAE
10X TBE
TE
20X SSC
LB medium
SOC medium
M9 Buffer
Lysis / IP Buffer
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